Optimal Peptide Storage: Temperatures and Durations
Complete guide on storage conditions for lyophilized and reconstituted peptides. Temperatures, shelf life and degradation factors.
Equipment required
Congélateur -20°C Réfrigérateur +4°C Dessiccant Flacons verre
Basic Principles
Peptides are sensitive molecules that can degrade through hydrolysis, oxidation or aggregation. Proper storage is essential to maintain their structural integrity.
Lyophilized Peptides (Powder)
Optimal Conditions
- Temperature: -20°C (standard freezer)
- Humidity: < 30% RH (use desiccant)
- Light: Protect from UV light
- Atmosphere: Under nitrogen if possible
Shelf Life
| Temperature | Typical Duration | Remarks |
|---|---|---|
| -80°C | 5+ years | Optimal for long-term storage |
| -20°C | 2-3 years | Standard laboratory |
| +4°C | 6-12 months | Short term only |
| Ambient | < 1 month | To avoid |
Reconstituted Peptides (Solution)
General Recommendations
Once reconstituted, the peptide is more vulnerable to degradation. Follow these guidelines:
- Storage: +2-8°C (refrigerator)
- Duration: Variable per peptide (7-30 days)
- Aliquoting: Divide into portions to avoid freeze-thaw cycles
Durations by Peptide Type
| Peptide | Duration at +4°C | Notes |
|---|---|---|
| BPC-157 | 14 days | Relatively stable |
| DSIP | 10 days | Hygroscopic |
| GHK-Cu | 30 days | Stabilized by copper |
| Semaglutide | 56 days | Very stable |
| NAD+ | 7 days | Light sensitive |
Degradation Factors
Hydrolysis
Cleavage of peptide bonds by water. Minimized by dry storage or in appropriate buffer.
Oxidation
Particularly affects methionine and cysteine residues. Use antioxidants or purge with nitrogen.
Aggregation
Formation of insoluble aggregates. Avoid high concentrations and thermal cycling.
Best Practices
- Never refreeze a reconstituted peptide
- Use borosilicate glass vials
- Label clearly with reconstitution date
- Visual inspection before use (precipitate, turbidity)